Publications

2017

Martin, AR, RA Pollack, A Capoferri, RF Ambinder, CM Durand, and RF Siliciano. 2017. Rapamycin-mediated mTOR inhibition uncouples HIV-1 latency reversal from cytokine-associated toxicity. J Clin Invest pii: 89552. doi: 10.1172/JCI89552. [Epub ahead of print].

Lusic, M, and RF Siliciano. 2017. Nuclear landscape of HIV-1 infection and integration. Nat Rev Microbiol 15:69-82.

2016

Hill, AL, DI Rosenbloom, E Goldstein, E Hanhauser, DR Kuritzkes, RF Siliciano, and TJ Henrich. 2016. Real-time predictions of reservoir size and rebound time during antiretroviral therapy interruption trials for HIV. PLoS Pathog 12:e1005535

Laskey, SB, and RF Siliciano. 2016. Quantitative evaluation of the antiretroviral efficacy of dolutegravir. JCI Insight 1:e90033.

Besnard, E, S Hakre, M Kampmann, HW Lim, NN Hosmane, A Martin, MC Bassik, E Verschueren, E Battivelli, J Chan, JP Svensson, A Gramatica, RJ Conrad, M Ott, WC Greene, NJ Krogan, RF Siliciano, JS Weissman, and E Verdin. 2016. The mTOR complex controls HIV latency. Cell Host Microbe 20:785-797.

Laird, GM, DI Rosenbloom, J Lai, RF Siliciano, and JD Siliciano. 2016. Measuring the frequency of latent HIV-1 in resting CD4⁺ T cells using a limiting dilution coculture assay. Methods Mol Biol 1354:239-253.

Hill, AL, DIS Rosenbloom, JD Siliciano, and RF Siliciano. 2016. Insufficient evidence for rare activation of latent HIV in the absence of reservoir-reducing interventions. PLoS Pathog 12:e1005679.

Sunshine, S, R Kirchner, SS Amr, L Mansur, R Shakhbatyan, M Kim, A Bosque, RF Siliciano, V Planelles, O Hofmann, S Ho Sui, and JZ Li. 2016. HIV integration site analysis of cellular models of HIV latency with a probe-enriched next-generation sequencing assay. J Virol 90:4511-4519.

Laskey, SB, CW Pohlmeyer, KM Bruner, and RF Siliciano. 2016. Evaluating clonal expansion of HIV-infected cells: optimization of pcr strategies to predict clonality. PLoS Pathog 12:e1005689.

Hansen, EC, M Ransom, JR Hesselberth, NN Hosmane, AA Capoferri, KM Bruner, RA Pollack, H Zhang, MB Drummond, JM Siliciano, R Siliciano, and JT Stivers. 2016. Diverse fates of uracilated HIV-1 DNA during infection of myeloid lineage cells. Elife 5:10.7554/eLife.18447.

Bruner, KM, AJ Murray, RA Pollack, MG Soliman, SB Laskey, AA Capoferri, J Lai, MC Strain, SM Lada, R Hoh, YC Ho, DD Richman, SG Deeks, JD Siliciano, and RF Siliciano. 2016. Defective proviruses rapidly accumulate during acute HIV-1 infection. Nat Med 22:1043-1049.

Murray, AJ, KJ Kwon, and RF Siliciano. 2016. The latent reservoir for HIV-1: How immunologic memory and clonal expansion contribute to HIV-1 persistence. J Immunol 197:407-17

Kim, M, and RF Siliciano. 2016. Reservoir expansion by T-cell proliferation may be another barrier to curing HIV infection. Proc Natl Acad Sci U S A 113:1692-1694.

Siliciano, JD, and RF Siliciano. 2016. Recent developments in the effort to cure HIV infection: going beyond N = 1. J Clin Invest 126:409-414.

Martin, AR, and RF Siliciano. 2016. Progress Toward HIV Eradication: Case Reports, Current Efforts, and the Challenges Associated with Cure. Annu Rev Med 67:215-228.

Kim, M, and RF Siliciano. 2016. Genome editing for clinical HIV isolates. Nat Biotechnol 34:388-389.

Churchill, MJ, SG Deeks, DM Margolis, RF Siliciano, and R Swanstrom. 2016. HIV reservoirs: what, where and how to target them. Nat Rev Microbiol 14:55-60.

2015

Zarr, M, and R Siliciano. 2015. Stoichiometric parameters of HIV-1 entry. Virology 474:1-9.

Gray, WT, KM Frey, SB Laskey, AC Mislak, KA Spasov, WG Lee, M Bollini, RF Siliciano, WL Jorgensen, and KS Anderson. 2015. Potent inhibitors active against HIV reverse transcriptase with K101P, a mutation conferring rilpivirine resistance. ACS Med Chem Lett 6:1075-1079.

Sampah, ME, GM Laird, JN Blankson, RF Siliciano, and JS Coleman. 2015. Medroxyprogesterone acetate increases HIV-1 infection of unstimulated peripheral blood mononuclear cells in vitro. AIDS 29:1137-1146.

Cohn, LB, IT Silva, TY Oliveira, RA Rosales, EH Parrish, GH Learn, BH Hahn, JL Czartoski, MJ McElrath, C Lehmann, F Klein, M Caskey, BD Walker, JD Siliciano, RF Siliciano, M Jankovic, and MC Nussenzweig. 2015. HIV-1 integration landscape during latent and active infection. Cell 160:420-432.

Laird, GM, CK Bullen, DI Rosenbloom, AR Martin, AL Hill, CM Durand, JD Siliciano, and RF Siliciano. 2015. Ex vivo analysis identifies effective HIV-1 latency-reversing drug combinations. J Clin Invest 125:1901-1912.

Rosenbloom, DI, O Elliott, AL Hill, TJ Henrich, JM Siliciano, and RF Siliciano. 2015. Designing and interpreting limiting dilution assays: general principles and applications to the latent reservoir for Human Immunodeficiency Virus-1. Open Forum Infect Dis 2:ofv123.

Deng, K, M Pertea, A Rongvaux, L Wang, CM Durand, G Ghiaur, J Lai, HL McHugh, H Hao, H Zhang, JB Margolick, C Gurer, AJ Murphy, DM Valenzuela, GD Yancopoulos, SG Deeks, T Strowig, P Kumar, JD Siliciano, SL Salzberg, RA Flavell, L Shan, and RF Siliciano. 2015. Broad CTL response is required to clear latent HIV-1 due to dominance of escape mutations. Nature 517:381-385.

Siliciano, JM, and RF Siliciano. 2015. The remarkable stability of the latent reservoir for HIV-1 in resting memory CD4+ T cells. J Infect Dis 212:1345-1347

Bruner, KM, NN Hosmane, and RF Siliciano. 2015. Towards an HIV-1 cure: measuring the latent reservoir. Trends Microbiol 23:192-203.

2014

Shan, L, S Xing, HC Yang, H Zhang, JB Margolick, and RF Siliciano. 2014. Unique characteristics of histone deacetylase inhibitors in reactivation of latent HIV-1 in Bcl-2-transduced primary resting CD4+ T cells. J Antimicrob Chemother 69:28-33.

Dar, RD, NN Hosmane, MR Arkin, RF Siliciano, and LS Weinberger. 2014. Screening for noise in gene expression identifies drug synergies. Science 344:1392-1396.

Kim, M, NN Hosmane, CK Bullen, A Capoferri, HC Yang, JD Siliciano, and RF Siliciano. 2014. A primary CD4⁺ T cell model of HIV-1 latency established after activation through the T cell receptor and subsequent return to quiescence. Nat Protoc 9:2755-2770.

Hill, AL, DI Rosenbloom, F Fu, MA Nowak, and RF Siliciano. 2014. Predicting the outcomes of treatment to eradicate the latent reservoir for HIV-1. Proc Natl Acad Sci USA 111:13475-80.

Spivak, AM, A Andrade, E Eisele, R Hoh, P Bacchetti, NN Bumpus, F Emad, R Buckheit 3rd, EF McCance-Katz, J Lai, M Kennedy, G Chander, RF Siliciano, JD Siliciano, and SG Deeks. 2014. A pilot study assessing the safety and latency-reversing activity of disulfiram in HIV-1-infected adults on antiretroviral therapy. Clin Infect Dis 58:883-890.

Laird, GM, EE Eisele, SA Rabi, D Nikolaeva, and RF Siliciano. 2014. A novel cell-based high-throughput screen for inhibitors of HIV-1 gene expression and budding identifies the cardiac glycosides. J Antimicrob Chemother 69:988-994.

Bullen, CK, GM Laird, CM Durand, JD Siliciano, and RF Siliciano. 2014. New ex vivo approaches distinguish effective and ineffective single agents for reversing HIV-1 latency in vivo. Nat Med 20:425-429.

Salgado, M, MD Swanson, CW Pohlmeyer, RW Buckheit 3rd, J Wu, NM Archin, TM Williams, DM Margolis, RF Siliciano, JV Garcia, and JN Blankson. 2014. HLA-B*57 elite suppressor and chronic progressor HIV-1 isolates replicate vigorously and cause CD4+ T cell depletion in humanized BLT mice. J Virol 88:3340-3352.

Cockerham, LR, JD Siliciano, E Sinclair, U O’Doherty, S Palmer, SA Yukl, MC Strain, N Chomont, FM Hecht, RF Siliciano, DD Richman, and SG Deeks. 2014. CD4+ and CD8+ T cell activation are associated with HIV DNA in resting CD4+ T cells. PLoS One 9:e110731.

Shan, L, and RF Siliciano. 2014. Unraveling the relationship between microbial translocation and systemic immune activation in HIV infection. J Clin Invest 124:2368-2371.

Siliciano, JD, and RF Siliciano. 2014. Recent developments in the search for a cure for HIV-1 infection: targeting the latent reservoir for HIV-1. J Allergy Clin Immunol 134:12-19.

Siliciano, RF. 2014. Opening Fronts in HIV Vaccine Development: Targeting reservoirs to clear and cure. Nat Med 20:480-481.

Laskey, SB, and RF Siliciano. 2014. A mechanistic theory to explain the efficacy of antiretroviral therapy. Nat Rev Microbiol 12:772-780.

Ho, YC, GM Laird, and RF Siliciano. 2014. Measuring reversal of HIV-1 latency ex vivo using cells from infected individuals. Proc Natl Acad Sci USA 111:6860-6861.

Cox, AL, and RF Siliciano. 2014. HIV: Not-so-innocent bystanders. Nature 505:492-493.

Deng, K, and RF Siliciano. 2014. HIV: Early treatment may not be early enough. Nature 512:35-36.

Blankson, JN, JD Siliciano, and RF Siliciano. 2014. Finding a cure for human immunodeficiency virus-1 infection. Infect Dis Clin North Am 28:633-650.

Durand, CM, and RF Siliciano. 2014. Dual zinc-finger nucleases block HIV infection. Blood 123:2-3.

Siliciano, JD, and RF Siliciano. 2014. AIDS/HIV. Rekindled HIV infection. Science 345:1005-1006.

2013

Ho, YC, L Shan, NN Hosmane, J Wang, SB Laskey, DI Rosenbloom, J Lai, JN Blankson, JD Siliciano, and RF Siliciano. 2013. Replication-competent noninduced proviruses in the latent reservoir increase barrier to HIV-1 cure. Cell 155:540-551.

2012

Shan, L, K Deng, NS Shroff, CM Durand, SA Rabi, HC Yang, H Zhang, JB Margolick, JN Blankson, and RF Siliciano. 2012. Stimulation of HIV-1-specific cytolytic T lymphocytes facilitates elimination of latent viral reservoir after virus reactivation. Immunity 36:491-501.

2011

Siliciano, RF, and WC Greene. 2011. HIV latency. Cold Spring Harb Perspect Med 1:a007096. PMCID:PMC3234450.

2009

Yang, HC, S Xing, L Shan, K O’Connell, J Dinoso, A Shen, Y Zhou, CK Shrum, Y Han, JO Liu, H Zhang, JB Margolick, and RF Siliciano. 2009. Small-molecule screening using a human primary cell model of HIV latency identifies compounds that reverse latency without cellular activation. J Clin Invest 119:3473-3486.

2006

Bailey, JR, AR Sedaghat, T Kieffer, T Brennan, PK Lee, M Wind-Rotolo, CM Haggerty, AR Kamireddi, Y Liu, J Lee, D Persaud, JE Gallant, J Cofrancesco Jr, TC Quinn, CO Wilke, SC Ray, JD Siliciano, RE Nettles, and RF Siliciano. 2006. Residual human immunodeficiency virus type 1 viremia in some patients on antiretroviral therapy is dominated by a small number of invariant clones rarely found in circulating CD4+ T cells. J Virol 80:6441-6457.

2003

Siliciano, JD, J Kajdas, D Finzi, TC Quinn, K Chadwick, JB Margolick, C Kovacs, SJ Gange, and RF Siliciano. 2003. Long-term follow-up studies confirm the stability of the latent reservoir for HIV-1 in resting CD4+ T cells. Nat Med 9:727-728.

1997

Finzi, D, M Hermankova, T Pierson, LM Carruth, C Buck, RE Chaisson, TC Quinn, K Chadwick, J Margolick, R Brookmeyer, J Gallant, M Markowitz, DD Ho, DD Richman, and RF Siliciano. 1997. Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy. Science 278:1295-1300.